Heterokaryosis and you will parasexual recombination in the pathogenic stresses away from Fusarium oxysponrm
V. Heterokaryosis and you can parasexuality
Utilize the “0”spot for one of the parents and notice the stress count into the plate. Use the theme towards replicator. Incubate 2-three days. Simulate the brand new segregants towards a few shot dishes having fun with a good replicator that have, elizabeth.grams., 21 needles. Draw this new dishes with a number. Incubate 2-three days. Rating the exam dishes and checklist the phenotypes regarding scoring desk. Just be sure to dictate brand new ploidy of your colonies to the base out of new indicators. Browse the ploidy away from unsure territories. Generate a list of the latest genotypes (you need to use a utility). Determine the latest percentage of the fresh recombinants to your more indicators. And that indicators is linked? Do you really discover intrachromosomal recombination? Where linkage classification is the not familiar marker?
Within this experiment we determine this new gene buy and location off brand new centromere inside the linkage category VI ofA. niger.Individuals approaches for your choice of mitotic recombinants are used. The fresh new markers on it is: pubA1, pyrB4, c d l . Brand new c d locus try terminal for the chromosome case and you will ergo really compatible as the options marker. Just like the every indicators was recessive, they should be in cis reputation. This new chlorate-unwilling segregants is isolated, as well as feel reviewed for the other markers. The brand new diploid used are: N761 N640
The newest diploid into MM, 4 dishes CMCIO3 A suspension system of conidiospores of good diploid colony step three dishes CM + C103, bottles with saline otherwise sterile drinking water step 3 dishes CM
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